Journal: Microbial Cell Factories

Article Title: Purification and partial characterization of bacillocin 490, a novel bacteriocin produced by a thermophilic strain of Bacillus licheniformi s

doi:

Figure Lengend Snippet: Activity spectrum of bacillocin 490

Article Snippet: Escherichia coli ATCC 13762 , -.

Techniques: Activity Assay

Journal: Plant Direct

Article Title: Arabidopsis MATE 45 antagonizes local abscisic acid signaling to mediate development and abiotic stress responses

doi: 10.1002/pld3.87

Figure Lengend Snippet: MATE 45 long has metabolite efflux activity. (a) Mutant Escherichia coli strain acrB that is defective in the Acr AB ‐TolC efflux system was transformed with ORF s MATE 45 long , MATE 45 med , MATE 45 short or yellow florescent protein (Control), and grown in the presence or absence of 1 mM of the toxin tetrabultylammonium ( TBA ). Bacterial growth absorbance is indicated on the vertical axis and time after initiating the culture is indicated on the horizontal axis. In the absence of toxin, all genotypes reached exponential growth at the same time, ensuring that equal numbers of bacteria were plated for each genotype. Only the MATE 45 long ORF provided tolerance to TBA toxin, as demonstrated by the failure to delay exponential growth. (b) acrB transformed with the MATE 45 long ORF or yellow florescent protein (Control) was grown in the presence or absence of different concentrations of the toxin TBA . MATE 45 long provided tolerance to the toxin, as demonstrated by earlier times until exponential phase growth in the present of the toxin. (c) mate45‐1 long lacked MATE efflux activity. acrB was transformed with the ORF mate45‐1 long , MATE 45 long , or yellow florescent protein (Control) and grown in the presence or absence of 1 mM TBA toxin. mate45‐1 long failed to provide tolerance to the TBA , as demonstrated by similar growth curve compared to the control

Article Snippet: The Escherichia coli mutant acrB deficient in drug efflux capability was obtained from the NBRP (National BioResource Project; National Institute of Genetics, Mishima, Japan).

Techniques: Activity Assay, Mutagenesis, Transformation Assay, Control, Bacteria

Journal: Plant Direct

Article Title: Arabidopsis MATE 45 antagonizes local abscisic acid signaling to mediate development and abiotic stress responses

doi: 10.1002/pld3.87

Figure Lengend Snippet: Ectopic localization of abscisic acid ( ABA ) signaling in mate45‐1 pDR 29B: GUS and (+)‐ ABA uptake. (a–d) Seedlings of wild‐type (a, c) and mate45‐1 (b, d) expressing the ABA signaling marker pDR 29B: GUS imaged six dag (a, b), and eight dag (c, d) in anthocyanin induction condition ( AIC ). Arrowheads mark regions of ectopic staining in mate45‐1 pRD 29B: GUS . Scale: 125 μm. (e) Response to ABA treatment. Seedlings grown in AIC for 5 days were treated with 1 μM ABA for 24 hr by application to the medium. Upper panels scale: 1 mm. Lower panels are enlargements of the boxed areas shown upper panels. Arrows indicated vasculature. mate45‐1 exhibited relatively weak staining in extravascular tissues of the hypocotyl and cotyledon. Scale: 250 μm. (f) Rate of (+)‐ ABA uptake into acrB cells expressing MATE 45long. Cells expressing MATE 45long (yellow bars) or Venus fluorescent protein as a non‐transporter control (black bars) were pelleted and resuspended in medium containing 0.5 μM (+)‐ ABA . After 0 or 30 min of uptake cells were re‐pelleted and extracted for analysis (+)‐ ABA amounts by LC ‐ MS in MRM mode. Bars represent the rate of (+)‐ ABA uptake calculated as the amount of (+)‐ ABA at T = 30 min subtracted by the amount adhered to the cells at 0 min divided by the 30 min incubation time. bG reater than control, p < 0.05; two‐tailed Student's t test

Article Snippet: The Escherichia coli mutant acrB deficient in drug efflux capability was obtained from the NBRP (National BioResource Project; National Institute of Genetics, Mishima, Japan).

Techniques: Expressing, Marker, Staining, Control, Liquid Chromatography with Mass Spectroscopy, Incubation, Two Tailed Test